identification of safflower as a fraud in commercial saffron using rapd/scar
نویسندگان
چکیده
saffron (crocus sativus) is the most valuable and indigenous crop in iran. the stigmas of flower are used as a popular natural flavouring, colouring and medicinal agent. however, the market suffers from frauds in this plant such as mixing with safflower petals due to high profit. identification of these frauds with conventional and biochemical methods is difficult and low sensitive. therefore, application of molecular markers such as random amplified polymorphic dna (rapd)/sequence characterized amplified regions (scar) is being considered as an alternative. in this study, dna was extracted from dry stigmas of 5 saffron accessions and dry petals of 7 safflower cultivars. rapd reactions with ten 15-mer random primers resulted in two specific monomorphic bands (500 and 700 bp) for safflower, while they were absent in saffron accessions. pcr analysis with specific scar primers amplified two specific bands (414 and 589 bp) for safflowers in different combinations of saffron stigmas and safflower petals. this was the case with very low rates or 1% of safflower. therefore, this method seems to be suitable for fraud identification of safflower petals in commercial saffron samples.
منابع مشابه
Studies on Commercial Saffron
Two commercial samples of saffron were analysed for moisture and ash contents, solubility in water, action with sulphuric acid, presence of foreign dyes and colour intensity of water soluble solution.The moisture content in Laccha was 12.7% while it was 11% in Mogra. The ash was 4.7% in both the samples. The water soluble matter in Laccha was 52.7% and 45.8% in Mogra. There was no foreign dye i...
متن کاملPyrosequencing as a tool for rapid fish species identification and commercial fraud detection.
The increased consumption of fish products, as well as the occurrence of exotic fish species in the Mediterranean Sea and in the fish market, has increased the risk of commercial fraud. Furthermore, the great amount of processed seafood products has greatly limited the application of classic identification systems. DNA-based identification allows a clear and unambiguous detection of polymorphis...
متن کاملthe significance of conjunction as a cohesive device in teaching writing
the research questions were as follows: 1. is there any relationship between the students concious awareness of the form and implications of the conjuncations and their improvement in using appropriate conjunctions? 2. does students knowledge of the from and the implications of the conjunctions help them to produce more coherent writings. 3. does a comparison between english conjunctions and th...
15 صفحه اولIdentification of Fraud in Banking Data and Financial Institutions Using Classification Algorithms
In recent years, due to the expansion of financial institutions,as well as the popularity of the World Wide Weband e-commerce, a significant increase in the volume offinancial transactions observed. In addition to the increasein turnover, a huge increase in the number of fraud by user’sabnormality is resulting in billions of dollars in lossesover the world. T...
متن کاملIdentification of Fraud in Banking Data and Financial Institutions Using Classification Algorithms
In recent years, due to the expansion of financial institutions,as well as the popularity of the World Wide Weband e-commerce, a significant increase in the volume offinancial transactions observed. In addition to the increasein turnover, a huge increase in the number of fraud by user’sabnormality is resulting in billions of dollars in lossesover the world. T...
متن کاملمنابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
journal of cell and molecular researchجلد ۳، شماره ۱، صفحات ۳۱-۰
میزبانی شده توسط پلتفرم ابری doprax.com
copyright © 2015-2023